Lehet, hogy neked vállalkozóként egyértelmű, hogy alvállalkozói díjról viszel be számlát a könyvelődnek, ő viszont egyszerűen igénybevett szolgáltatásnak könyveli.
Ez azért nem jó, mert amíg az alvállalkozói díjakat levonhatod az iparűzési adó alapjából, az igénybevett szolgáltatást nem. Százezreket bukhatsz, ha nem vagy körültekintő.
Az iparűzési adó alapja társaságoknál a nettó árbevétel.
Ezt lehet csökkenteni az alábbiakkal:
- eladott áruk beszerzési értékének valamint
- közvetített szolgáltatások arányosított értékével
- alvállalkozói teljesítések értékével
- anyagköltséggel
Amint látod, az igénybevett szolgáltatással nem.
Márpedig, ha egy számlában foglalt szolgáltatás nem felel az alvállalkozói díjról kiállított számla feltételeinek, akkor könyvelőként kénytelenek vagyunk azt igénybevett szolgáltatásként kezelni.
A pontosság kedvéért lássuk tehát, hogy mit értünk alvállalkozói díjon:
Alvállalkozói teljesítmény alatt azt a tevékenységet értjük, amelyről a vállalkozó a megrendelővel és az alvállalkozóval is a Ptk. szerinti vállalkozási szerződést kötött. Figyelni kell a vállalkozási szerződésre. Tényleg az legyen. Érdemes már a megrendelővel kötött szerződésben utalni arra, hogy alvállalkozóval végezteted a munkát.
Ha ezek a szerződések – megrendelések, teljesítés igazolások – hiányoznak, illetve nem derül ki a számlából, hogy azt a megrendelővel kötött szerződéseddel kapcsolatos munkára vetted igénybe, akkor sajnos nem lehet alvállalkozói díjként figyelembe venni.
Javaslom, hogy ha esetleg sokallod az iparűzési adód összegét, akkor beszélj a könyvelőddel, hátha tudod pótolni a szerződéseket és át lehet könyvelni ezeket a számlákat, hogy figyelembe vehető legyen.
Ilyen és hasonló témákat beszélünk meg 2018. április 14-én a társaságok ügyvezetőinek szóló 2 órás tréningünkön.
Ha érdekel, hogyan tudod elkerülni az adóbírságokat, akkor nézd meg az alábbi linken a témákat és jelentkezz az előadásra!
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(Master of Business Administration, master of business administration) is not so much a training program as a Manager’s degree (in management). Here it is important to have it, which indicates that you have the skills necessary for a middle-and senior-level Manager. To date, there is no single standard that would allow you to divide the degree of proficiency in these skills, so only the fact of having a corresponding diploma is taken into account.
The first version of such a training system began to be used in the United States in the 19th century. for many companies of that time, employees who knew the latest management methods were in demand. With
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Getting first-hand knowledge from world-class businessmen, and perhaps even from Nobel prize winners.
A great opportunity to get a job abroad or in our country, but in an international organization.
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We lived in a provincial town. I was very surprised that before us in a neighboring school from the 8th grade, parents took their son to family training. My father taught me. The headmaster of that school proudly announced that the boy returned a year later: “It was hard.” The boy pulled it out, passed it well, but didn’t want to do it again.
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What happened to the first boy in family training in our city is the first pitfall. We came across it in the 5th grade. This is the desire of teachers to interrogate (do not ask!) a non-standard student on all issues. No student answers on all topics in all subjects. Only those who are in family training.
I was acutely aware of the teachers ‘ desire to prove that the system they serve is better. So that others don’t get hurt. We found a way out: another school.
Choose the form of training: external. They will impose fewer requirements.
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My mother-in-law worked as a teacher all her life. The students loved her. She took our idea of home education badly. We were lucky to live in different cities.
Once she arrived for 2 days and, as it turned out, did not have time to go to school! I wanted to talk to the class teacher, so that the girl was asked more strictly, so that she didn’t want to study at home.
It was a failed betrayal. Sticks in the wheels can be inserted by anyone who does not feel sorry for the child. Such people were always on the way. But my own grandmother…
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My parents didn’t agree with our decision either. However, the grandfather wrote to his granddaughter: “Don’t see that your mother and I disagree. Listen to your mother, no one loves you more than her.”
Detractors are the second pitfall. Inevitable. The worst thing is when it’s relatives.
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Natasha finished 10th and 11th grade in one year. 3 times I wanted to drop out of school. Overwork. What held her back was the thought of those who would also study as an external student after her. She knew that she was paving the wa
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Home education pitfalls.
We lived in a provincial town. I was very surprised that before us in a neighboring school from the 8th grade, parents took their son to family training. My father taught me. The headmaster of that school proudly announced that the boy returned a year later: “It was hard.” The boy pulled it out, passed it well, but didn’t want to do it again.
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The first pitfall
What happened to the first boy in family training in our city is the first pitfall. We came across it in the 5th grade. This is the desire of teachers to interrogate (do not ask!) a non-standard student on all issues. No student answers on all topics in all subjects. Only those who are in family training.
I was acutely aware of the teachers ‘ desire to prove that the system they serve is better. So that others don’t get hurt. We found a way out: another school.
Choose the form of training: external. They will impose fewer requirements.
The second pitfall
My mother-in-law worked as a teacher all her life. The students loved her. She took our idea of home education badly. We were lucky to live in different cities.
Once she arrived for 2 days and, as it turned out, did not have time to go to school! I wanted to talk to the class teacher, so that the girl was asked more strictly, so that she didn’t want to study at home.
It was a failed betrayal. Sticks in the wheels can be inserted by anyone who does not feel sorry for the child. Such people were always on the way. But my own grandmother…
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My parents didn’t agree with our decision either. However, the grandfather wrote to his granddaughter: “Don’t see that your mother and I disagree. Listen to your mother, no one loves you more than her.”
Detractors are the second pitfall. Inevitable. The worst thing is when it’s relatives.
The third pitfall
Natasha finished 10th and 11th grade in one year. 3 times I wanted to drop out of school. Overwork. What held her back was the thought of those who would also study as an external student after her. She knew that she was paving the wa
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Like any other ability, memory can be trained and developed. The main thing is to choose the right exercise program.
Remembering the name of an actor or finding the keys left in the evening can sometimes be difficult. It takes a lot of effort to recover the information you need. Forgetting is a natural process that is designed to unload the nervous system from unclaimed information, to protect it from overstrain. But increased forgetfulness can significantly complicate life. It can be caused by lack of sleep, stress, addictions, or information overload.
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It is not necessary to take the shopping list prepared before going to the store. On the one hand, such a list will help not to miss anything and at the same time not to buy too much. On the other hand, its absence will have a positive effect on the state of memory. Do not buy something this time – by the next trip you will have an incentive to better memorize items from the list and keep them in mind. Forgot someone’s name, phone number, password or recipe ingredients? Try to remember. Take the time and take a few minutes to do this instead of looking for an answer in your smartphone or notebook. This is great for strengthening memory.
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Another way to activate memory is to plunge into the past day and remember all its events. Before going to bed, try to reconstruct snippets of dialogue, images of people, their facial expressions and gestures, every phone call and meals that made up your diet. Try to recreate the sequence of all actions from the moment of awakening. Provide these pictures in as much detail as possible. At the same time, it is important to connect imagination, visual, auditory and olfactory memory.
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The structure of the thesis According to the generally accepted rules for writing a diploma, its main part should consist of three chapters: First (at least three paragraphs). Written on the basis of a synthesis and analysis of theoretical information gleaned from selected sources; reveals the central concepts, the essence of a phenomenon or process, different points of view on the object and subject of research. Second (at least three subsections). Assigned to factual material and analysis of the problem; contains statistical information with quantitative and qualitative characteristics of the phenomenon under consideration, a statement of the identified trends and shortcomings in its state and development. Third (the minimum number of paragraphs is three). Dedicated to developing a solution to a problem. His diploma student proposes on the basis of the second chapter with scientific justification, for which the theoretical provisions of the first are involved. The chapters should be approximately equal in length. At the final stage, a conclusion, an introduction, a final list of used literature are drawn up and a block with annexes is completed. How to properly draw up a thesis In student memos and on their official websites, all universities must post detailed methodological recommendations containing requirements for a diploma. They indicate: volume – how many pages in the diploma should be; structure – how many chapters to divide the study, what to write in the introduction and conclusion; what materials should be included in the attachments and how to number them; technical regulations – where to put down, from which sheet to start pagination, what font to use for text and headings, how to indent the paragraph and margins, line spacing; the number of sources in the bibliographic list; permissible percentage of uniqueness when checking for plagiarism. Most universities and institutes include in methodological collections: a template for the title page; forms of reviews, annotations, reviews; sample bibliography; examples of the design of references, footnotes, formulas, tables, figures, diagrams, photos. It remains only to carefully check with the university standard and follow the recommendations, since the normative control will be meticulously carried out precisely according to the points of the manual, and only then relying on GOSTs. Average requirements for a thesis: Volume – 50-70 pages, excluding attachments and, as a rule, bibliography. The number of sources is at least 40, and 70% are publications not older than five years. The bibliographic list is drawn up in accordance with GOST 7.80-2000, 7.1-2003, 7.0.5-2008, 7.0.1-2011. Times New Roman in black, 14th for text, 15th for subheadings, 16th for headings. The interval is 1.5. Margins – at least 2 cm to the left, bottom and top; 1.5 cm – on the right. Printing is only one-sided. The standard of uniqueness of the diploma: humanities – 80%, exact – 75%.
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The next stage is complex and consists in defining goals, objectives, research methods, selection and systematization of relevant literature and parallel drawing up of a thesis plan, which is a preliminary outline of the structure and content of future student scientific work. When familiarizing with the sources of information, one should take into account their novelty. The periods of obsolescence of educational literature are established by order of the Ministry of Education and Science No. 1623 of April 11, 2001: for a general humanitarian, socio-economic profile and special disciplines – five years; for general professional, natural science, mathematical subjects – ten years. The measures of relevance do not relate to sources of in-depth fundamental study of the issue. It does not stipulate the order and terms after which research publications: articles, dissertations, reports, etc., are considered outdated, but university regulatory controllers, when checking their thesis for compliance with standards, are almost always guided by the requirements set for textbooks. For informational purposes, no one forbids using them, but in order to avoid sending the finished diploma for revision, it is better to include only works no older than five years in the list of used literature. In January 2017, the order of the Ministry of Education and Science entered into force No. 1651, canceling the effect of orders No. 1623 and No. 133, which regulated the provision of university libraries with scientific and educational literature (it was in them that the terms of its obsolescence were prescribed). The new document aroused a lot of questions from the experts of the academic community, and the official answers of the department to them did not clarify the situation, so teachers still prefer to adhere to the old standards, determining the “expiration date” of the sources used to write the thesis. Algorithm of writing The scheme of work on a diploma is standard for all students – according to a single algorithm, graduates who write both project and research FQPs go to future defense. First of all, you need to decide on a supervisor, then choose a topic. It is approved before the start of the pre-diploma practice, to which the student is sent with the assignment for the FQP signed by the head of the department.
The structure of the thesis According to the generally accepted rules for writing a diploma, its main part should consist of three chapters: First (at least three paragraphs). Written on the basis of a synthesis and analysis of theoretical information gleaned from selected sources; reveals the central concepts, the essence of a phenomenon or process, different points of view on the object and subject of research. Second (at least three subsections). Assigned to factual material and analysis of the problem; contains statistical information with quantitative and qualitative characteristics of the phenomenon under consideration, a statement of the identified trends and shortcomings in its state and development. Third (the minimum number of paragraphs is three). Dedicated to developing a solution to a problem. His diploma student proposes on the basis of the second chapter with scientific justification, for which the theoretical provisions of the first are involved. The chapters should be approximately equal in length. At the final stage, a conclusion, an introduction, a final list of used literature are drawn up and a block with annexes is completed. How to properly draw up a thesis In student memos and on their official websites, all universities must post detailed methodological recommendations containing requirements for a diploma. They indicate: volume – how many pages in the diploma should be; structure – how many chapters to divide the study, what to write in the introduction and conclusion; what materials should be included in the attachments and how to number them; technical regulations – where to put down, from which sheet to start pagination, what font to use for text and headings, how to indent the paragraph and margins, line spacing; the number of sources in the bibliographic list; permissible percentage of uniqueness when checking for plagiarism. Most universities and institutes include in methodological collections: a template for the title page; forms of reviews, annotations, reviews; sample bibliography; examples of the design of references, footnotes, formulas, tables, figures, diagrams, photos. It remains only to carefully check with the university standard and follow the recommendations, since the normative control will be meticulously carried out precisely according to the points of the manual, and only then relying on GOSTs. Average requirements for a thesis: Volume – 50-70 pages, excluding attachments and, as a rule, bibliography. The number of sources is at least 40, and 70% are publications not older than five years. The bibliographic list is drawn up in accordance with GOST 7.80-2000, 7.1-2003, 7.0.5-2008, 7.0.1-2011. Times New Roman in black, 14th for text, 15th for subheadings, 16th for headings. The interval is 1.5. Margins – at least 2 cm to the left, bottom and top; 1.5 cm – on the right. Printing is only one-sided. The standard of uniqueness of the diploma: humanities – 80%, exact – 75%.
How to choose a company for writing a diploma Checklist: A solid site – it should be a kind of Internet branch of an officially registered company with at least one offline office; that is, the site must be a full-fledged working tool, and not a one-page business card on free hosting. The age of the company is at least 5 years. Only those who really know how to work can become a “veteran” of the market. Conclusion of an agreement – the lists of customers of the company for writing student papers are not transferred to the dean’s offices, do not worry. You need a confirmation of the deal – to ensure that the work is done.
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Otherwise, it will be difficult to find aspects that are not covered by anyone and, on the contrary, to select materials for development, analysis, argumentation, comparison.
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The next stage is complex and consists in defining goals, objectives, research methods, selection and systematization of relevant literature and parallel drawing up of a thesis plan, which is a preliminary outline of the structure and content of future student scientific work. When familiarizing with the sources of information, one should take into account their novelty. The periods of obsolescence of educational literature are established by order of the Ministry of Education and Science No. 1623 of April 11, 2001: for a general humanitarian, socio-economic profile and special disciplines – five years; for general professional, natural science, mathematical subjects – ten years. The measures of relevance do not relate to sources of in-depth fundamental study of the issue. It does not stipulate the order and terms after which research publications: articles, dissertations, reports, etc., are considered outdated, but university regulatory controllers, when checking their thesis for compliance with standards, are almost always guided by the requirements set for textbooks. For informational purposes, no one forbids using them, but in order to avoid sending the finished diploma for revision, it is better to include only works no older than five years in the list of used literature. In January 2017, the order of the Ministry of Education and Science entered into force No. 1651, canceling the effect of orders No. 1623 and No. 133, which regulated the provision of university libraries with scientific and educational literature (it was in them that the terms of its obsolescence were prescribed). The new document aroused a lot of questions from the experts of the academic community, and the official answers of the department to them did not clarify the situation, so teachers still prefer to adhere to the old standards, determining the “expiration date” of the sources used to write the thesis. Algorithm of writing The scheme of work on a diploma is standard for all students – according to a single algorithm, graduates who write both project and research FQPs go to future defense. First of all, you need to decide on a supervisor, then choose a topic. It is approved before the start of the pre-diploma practice, to which the student is sent with the assignment for the FQP signed by the head of the department.
The structure of the thesis According to the generally accepted rules for writing a diploma, its main part should consist of three chapters: First (at least three paragraphs). Written on the basis of a synthesis and analysis of theoretical information gleaned from selected sources; reveals the central concepts, the essence of a phenomenon or process, different points of view on the object and subject of research. Second (at least three subsections). Assigned to factual material and analysis of the problem; contains statistical information with quantitative and qualitative characteristics of the phenomenon under consideration, a statement of the identified trends and shortcomings in its state and development. Third (the minimum number of paragraphs is three). Dedicated to developing a solution to a problem. His diploma student proposes on the basis of the second chapter with scientific justification, for which the theoretical provisions of the first are involved. The chapters should be approximately equal in length. At the final stage, a conclusion, an introduction, a final list of used literature are drawn up and a block with annexes is completed. How to properly draw up a thesis In student memos and on their official websites, all universities must post detailed methodological recommendations containing requirements for a diploma. They indicate: volume – how many pages in the diploma should be; structure – how many chapters to divide the study, what to write in the introduction and conclusion; what materials should be included in the attachments and how to number them; technical regulations – where to put down, from which sheet to start pagination, what font to use for text and headings, how to indent the paragraph and margins, line spacing; the number of sources in the bibliographic list; permissible percentage of uniqueness when checking for plagiarism. Most universities and institutes include in methodological collections: a template for the title page; forms of reviews, annotations, reviews; sample bibliography; examples of the design of references, footnotes, formulas, tables, figures, diagrams, photos. It remains only to carefully check with the university standard and follow the recommendations, since the normative control will be meticulously carried out precisely according to the points of the manual, and only then relying on GOSTs. Average requirements for a thesis: Volume – 50-70 pages, excluding attachments and, as a rule, bibliography. The number of sources is at least 40, and 70% are publications not older than five years. The bibliographic list is drawn up in accordance with GOST 7.80-2000, 7.1-2003, 7.0.5-2008, 7.0.1-2011. Times New Roman in black, 14th for text, 15th for subheadings, 16th for headings. The interval is 1.5. Margins – at least 2 cm to the left, bottom and top; 1.5 cm – on the right. Printing is only one-sided. The standard of uniqueness of the diploma: humanities – 80%, exact – 75%.
How to choose a company for writing a diploma Checklist: A solid site – it should be a kind of Internet branch of an officially registered company with at least one offline office; that is, the site must be a full-fledged working tool, and not a one-page business card on free hosting. The age of the company is at least 5 years. Only those who really know how to work can become a “veteran” of the market. Conclusion of an agreement – the lists of customers of the company for writing student papers are not transferred to the dean’s offices, do not worry. You need a confirmation of the deal – to ensure that the work is done.
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According to a recent study, lack of physical activity is the main risk factor for premature mortality for the entire world population.
This paper highlights the percentage of deaths that can be attributed to sedentary lifestyles worldwide. An alarming conclusion is that a sedentary lifestyle is the main risk factor for many non-communicable diseases. These include cardiovascular disease, coronary artery disease, stroke, hypertension, and type 2 diabetes. There are also cancers (bladder, stomach, kidney, breast, colon, and esophagus), depression, and dementia.
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A study published in the British Medical Journal of Sports Medicine on March 29, 2021, estimates that physical inactivity is responsible for 7.2% of all-cause deaths each year. This represents about 4 million deaths out of an average of 56.9 million per year. The American and Canadian researchers behind this work obtained information from several databases in different states. In addition, they tried to get the results closest to reality by considering pathologies whose causal relationship with a sedentary lifestyle is well known. In other words, we are talking about a serious level of evidence. According to the results, in developing countries, most deaths (in absolute numbers) occur due to the lack of physical activity of a part of their population. On the other hand, developed countries are more likely to suffer from the effects of a sedentary lifestyle, as they are subject to a much higher percentage of deaths. The study’s authors strongly believe that ” the public health burden associated with physical inactivity is a global problem that will require international cooperation to mobilize change and achieve these public health goals.” For researchers, change should be focused on achieving public health goals. This means increased investment in the re-opening of sports facilities during the pandemic. It also means making them available to as many people as possible in the long run.
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Sedentary lifestyle claims more than 4 million direct victims each year
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According to a recent study, lack of physical activity is the main risk factor for premature mortality for the entire world population.
This paper highlights the percentage of deaths that can be attributed to sedentary lifestyles worldwide. An alarming conclusion is that a sedentary lifestyle is the main risk factor for many non-communicable diseases. These include cardiovascular disease, coronary artery disease, stroke, hypertension, and type 2 diabetes. There are also cancers (bladder, stomach, kidney, breast, colon, and esophagus), depression, and dementia.
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A study published in the British Medical Journal of Sports Medicine on March 29, 2021, estimates that physical inactivity is responsible for 7.2% of all-cause deaths each year. This represents about 4 million deaths out of an average of 56.9 million per year. The American and Canadian researchers behind this work obtained information from several databases in different states. In addition, they tried to get the results closest to reality by considering pathologies whose causal relationship with a sedentary lifestyle is well known. In other words, we are talking about a serious level of evidence. According to the results, in developing countries, most deaths (in absolute numbers) occur due to the lack of physical activity of a part of their population. On the other hand, developed countries are more likely to suffer from the effects of a sedentary lifestyle, as they are subject to a much higher percentage of deaths. The study’s authors strongly believe that ” the public health burden associated with physical inactivity is a global problem that will require international cooperation to mobilize change and achieve these public health goals.” For researchers, change should be focused on achieving public health goals. This means increased investment in the re-opening of sports facilities during the pandemic. It also means making them available to as many people as possible in the long run.
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Sedentary lifestyle claims more than 4 million direct victims each year
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According to a recent study, lack of physical activity is the main risk factor for premature mortality for the entire world population.
This paper highlights the percentage of deaths that can be attributed to sedentary lifestyles worldwide. An alarming conclusion is that a sedentary lifestyle is the main risk factor for many non-communicable diseases. These include cardiovascular disease, coronary artery disease, stroke, hypertension, and type 2 diabetes. There are also cancers (bladder, stomach, kidney, breast, colon, and esophagus), depression, and dementia.
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A study published in the British Medical Journal of Sports Medicine on March 29, 2021, estimates that physical inactivity is responsible for 7.2% of all-cause deaths each year. This represents about 4 million deaths out of an average of 56.9 million per year. The American and Canadian researchers behind this work obtained information from several databases in different states. In addition, they tried to get the results closest to reality by considering pathologies whose causal relationship with a sedentary lifestyle is well known. In other words, we are talking about a serious level of evidence. According to the results, in developing countries, most deaths (in absolute numbers) occur due to the lack of physical activity of a part of their population. On the other hand, developed countries are more likely to suffer from the effects of a sedentary lifestyle, as they are subject to a much higher percentage of deaths. The study’s authors strongly believe that ” the public health burden associated with physical inactivity is a global problem that will require international cooperation to mobilize change and achieve these public health goals.” For researchers, change should be focused on achieving public health goals. This means increased investment in the re-opening of sports facilities during the pandemic. It also means making them available to as many people as possible in the long run.
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Sedentary lifestyle claims more than 4 million direct victims each year
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According to a recent study, lack of physical activity is the main risk factor for premature mortality for the entire world population.
This paper highlights the percentage of deaths that can be attributed to sedentary lifestyles worldwide. An alarming conclusion is that a sedentary lifestyle is the main risk factor for many non-communicable diseases. These include cardiovascular disease, coronary artery disease, stroke, hypertension, and type 2 diabetes. There are also cancers (bladder, stomach, kidney, breast, colon, and esophagus), depression, and dementia.
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A study published in the British Medical Journal of Sports Medicine on March 29, 2021, estimates that physical inactivity is responsible for 7.2% of all-cause deaths each year. This represents about 4 million deaths out of an average of 56.9 million per year. The American and Canadian researchers behind this work obtained information from several databases in different states. In addition, they tried to get the results closest to reality by considering pathologies whose causal relationship with a sedentary lifestyle is well known. In other words, we are talking about a serious level of evidence. According to the results, in developing countries, most deaths (in absolute numbers) occur due to the lack of physical activity of a part of their population. On the other hand, developed countries are more likely to suffer from the effects of a sedentary lifestyle, as they are subject to a much higher percentage of deaths. The study’s authors strongly believe that ” the public health burden associated with physical inactivity is a global problem that will require international cooperation to mobilize change and achieve these public health goals.” For researchers, change should be focused on achieving public health goals. This means increased investment in the re-opening of sports facilities during the pandemic. It also means making them available to as many people as possible in the long run.
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Stress often causes bacteria to form biofilms. Stress can manifest as a physical barrier, ultraviolet light, or a toxic substance such as antibiotics. The formation of these biofilms takes from several hours to days, and they can be of different shapes, sizes, colors, and textures depending on the type of bacteria. Being in a biofilm state protects them from harmful substances in the environment – biofilms have a unique outer wall with different physical and chemical properties than their individual cells. They can coordinate metabolism, slow down their growth, and even form an impenetrable barrier of wrinkles and creases. This is one of the ways they achieve high antibiotic resistance. Researchers from the United Kingdom recently studied the transition of the Hay Bacillus bacterium from a free-moving swarm to a biofilm as a defense mechanism and published what they did to combat its antibiotic-resistant properties in eLife.
To determine if their test strain was behaving like the others, they first ran stress tests on them. They tested the bacteria’s response to the physical barrier, ultraviolet light, and antibiotics. The addition of a physical barrier led to the transition of bacteria from one layer to a multilayer layer, followed by an increase in cell density and the formation of multilayer islands near the barrier. Later, wrinkles formed on the islands near the barrier in the place where they began to appear initially.
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When they applied ultraviolet light to the swarm, they again observed a drop in cell speed and an increase in density. And after the scientists added a large dose of the antibiotic kanamycin, the bacterial cells formed a biofilm. The researchers then developed a strategy to combat this bacterial biofilm. They added kanamycin to the environment of a new batch of swarming bacterial cells and watched as the biofilm began to form. They then re-injected the antibiotic at a much higher dose than the first, just before the biofilm formation was complete. As a result, the partially formed biofilm was destroyed and bacterial cells died. This shows that antibiotic-resistant bacteria lose their resistance to antibiotics when they undergo a phase transition, right before going into the biofilm, where they will become much more resistant. Thus, with the correct administration of antibiotics, the bacteria can be attacked in their most vulnerable state and destroyed. The researchers believe that similar transitions from swarm to biofilm occur in other bacterial species. Their research may pave the way for finding more effective ways to control clinically relevant bacteria. For example, Salmonella enterica, which spreads into the bloodstream and is transmitted through contaminated food. Or Pseudomonas aeruginosa with multiple drug resistance, which after surgery causes infections in the blood, lungs (pneumonia) and other parts of the body and spreads in hospitals.
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Stress often causes bacteria to form biofilms. Stress can manifest as a physical barrier, ultraviolet light, or a toxic substance such as antibiotics. The formation of these biofilms takes from several hours to days, and they can be of different shapes, sizes, colors, and textures depending on the type of bacteria. Being in a biofilm state protects them from harmful substances in the environment – biofilms have a unique outer wall with different physical and chemical properties than their individual cells. They can coordinate metabolism, slow down their growth, and even form an impenetrable barrier of wrinkles and creases. This is one of the ways they achieve high antibiotic resistance. Researchers from the United Kingdom recently studied the transition of the Hay Bacillus bacterium from a free-moving swarm to a biofilm as a defense mechanism and published what they did to combat its antibiotic-resistant properties in eLife.
To determine if their test strain was behaving like the others, they first ran stress tests on them. They tested the bacteria’s response to the physical barrier, ultraviolet light, and antibiotics. The addition of a physical barrier led to the transition of bacteria from one layer to a multilayer layer, followed by an increase in cell density and the formation of multilayer islands near the barrier. Later, wrinkles formed on the islands near the barrier in the place where they began to appear initially.
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When they applied ultraviolet light to the swarm, they again observed a drop in cell speed and an increase in density. And after the scientists added a large dose of the antibiotic kanamycin, the bacterial cells formed a biofilm. The researchers then developed a strategy to combat this bacterial biofilm. They added kanamycin to the environment of a new batch of swarming bacterial cells and watched as the biofilm began to form. They then re-injected the antibiotic at a much higher dose than the first, just before the biofilm formation was complete. As a result, the partially formed biofilm was destroyed and bacterial cells died. This shows that antibiotic-resistant bacteria lose their resistance to antibiotics when they undergo a phase transition, right before going into the biofilm, where they will become much more resistant. Thus, with the correct administration of antibiotics, the bacteria can be attacked in their most vulnerable state and destroyed. The researchers believe that similar transitions from swarm to biofilm occur in other bacterial species. Their research may pave the way for finding more effective ways to control clinically relevant bacteria. For example, Salmonella enterica, which spreads into the bloodstream and is transmitted through contaminated food. Or Pseudomonas aeruginosa with multiple drug resistance, which after surgery causes infections in the blood, lungs (pneumonia) and other parts of the body and spreads in hospitals.
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Stress often causes bacteria to form biofilms. Stress can manifest as a physical barrier, ultraviolet light, or a toxic substance such as antibiotics. The formation of these biofilms takes from several hours to days, and they can be of different shapes, sizes, colors, and textures depending on the type of bacteria. Being in a biofilm state protects them from harmful substances in the environment – biofilms have a unique outer wall with different physical and chemical properties than their individual cells. They can coordinate metabolism, slow down their growth, and even form an impenetrable barrier of wrinkles and creases. This is one of the ways they achieve high antibiotic resistance. Researchers from the United Kingdom recently studied the transition of the Hay Bacillus bacterium from a free-moving swarm to a biofilm as a defense mechanism and published what they did to combat its antibiotic-resistant properties in eLife.
To determine if their test strain was behaving like the others, they first ran stress tests on them. They tested the bacteria’s response to the physical barrier, ultraviolet light, and antibiotics. The addition of a physical barrier led to the transition of bacteria from one layer to a multilayer layer, followed by an increase in cell density and the formation of multilayer islands near the barrier. Later, wrinkles formed on the islands near the barrier in the place where they began to appear initially.
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When they applied ultraviolet light to the swarm, they again observed a drop in cell speed and an increase in density. And after the scientists added a large dose of the antibiotic kanamycin, the bacterial cells formed a biofilm. The researchers then developed a strategy to combat this bacterial biofilm. They added kanamycin to the environment of a new batch of swarming bacterial cells and watched as the biofilm began to form. They then re-injected the antibiotic at a much higher dose than the first, just before the biofilm formation was complete. As a result, the partially formed biofilm was destroyed and bacterial cells died. This shows that antibiotic-resistant bacteria lose their resistance to antibiotics when they undergo a phase transition, right before going into the biofilm, where they will become much more resistant. Thus, with the correct administration of antibiotics, the bacteria can be attacked in their most vulnerable state and destroyed. The researchers believe that similar transitions from swarm to biofilm occur in other bacterial species. Their research may pave the way for finding more effective ways to control clinically relevant bacteria. For example, Salmonella enterica, which spreads into the bloodstream and is transmitted through contaminated food. Or Pseudomonas aeruginosa with multiple drug resistance, which after surgery causes infections in the blood, lungs (pneumonia) and other parts of the body and spreads in hospitals.
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Stress often causes bacteria to form biofilms. Stress can manifest as a physical barrier, ultraviolet light, or a toxic substance such as antibiotics. The formation of these biofilms takes from several hours to days, and they can be of different shapes, sizes, colors, and textures depending on the type of bacteria. Being in a biofilm state protects them from harmful substances in the environment – biofilms have a unique outer wall with different physical and chemical properties than their individual cells. They can coordinate metabolism, slow down their growth, and even form an impenetrable barrier of wrinkles and creases. This is one of the ways they achieve high antibiotic resistance. Researchers from the United Kingdom recently studied the transition of the Hay Bacillus bacterium from a free-moving swarm to a biofilm as a defense mechanism and published what they did to combat its antibiotic-resistant properties in eLife.
To determine if their test strain was behaving like the others, they first ran stress tests on them. They tested the bacteria’s response to the physical barrier, ultraviolet light, and antibiotics. The addition of a physical barrier led to the transition of bacteria from one layer to a multilayer layer, followed by an increase in cell density and the formation of multilayer islands near the barrier. Later, wrinkles formed on the islands near the barrier in the place where they began to appear initially.
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When they applied ultraviolet light to the swarm, they again observed a drop in cell speed and an increase in density. And after the scientists added a large dose of the antibiotic kanamycin, the bacterial cells formed a biofilm. The researchers then developed a strategy to combat this bacterial biofilm. They added kanamycin to the environment of a new batch of swarming bacterial cells and watched as the biofilm began to form. They then re-injected the antibiotic at a much higher dose than the first, just before the biofilm formation was complete. As a result, the partially formed biofilm was destroyed and bacterial cells died. This shows that antibiotic-resistant bacteria lose their resistance to antibiotics when they undergo a phase transition, right before going into the biofilm, where they will become much more resistant. Thus, with the correct administration of antibiotics, the bacteria can be attacked in their most vulnerable state and destroyed. The researchers believe that similar transitions from swarm to biofilm occur in other bacterial species. Their research may pave the way for finding more effective ways to control clinically relevant bacteria. For example, Salmonella enterica, which spreads into the bloodstream and is transmitted through contaminated food. Or Pseudomonas aeruginosa with multiple drug resistance, which after surgery causes infections in the blood, lungs (pneumonia) and other parts of the body and spreads in hospitals.
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